Journal article
Chemical modification studies on protein synthesis inhibitor II from barley seeds. Identification of an essential tyrosyl residue
An inhibitor of animal cell-free protein synthesis from barley seeds (inhibitor II) has been chemically modified by means of group-specific reagents. Specific nitration of a single tyrosyl residue caused a substantial loss of the inhibitory activity. After cyanogen bromide cleavage and subfragmentation of the nitrated fragment by endoproteinase Lys-C, the essential residue was identified as Tyr(86) in the primary structure.
Chemical modifications of carboxyl groups, lysyl residues, and arginyl residues also led to reduction of the activity, whereas modifications of tryptophanyl or methionyl residues had no significant effects.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 1986 |
Pages: | 501-507 |
ISSN: | 01051938 |
Types: | Journal article |
DOI: | 10.1007/BF02906892 |