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Journal article

Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

By Allan, G.M.; Mc Neilly, F.; Meehan, B.M.; Kennedy, S.; Mackie, D.P.; Ellis, J.A.; Clark, E.G.; Espuna, E.; Saubi, N.; Riera, P.; Bøtner, Anette1,2,3; Charreyre, C.E. ...and 2 more

From

Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark1

Division of Virology, National Veterinary Institute, Technical University of Denmark2

National Veterinary Institute, Technical University of Denmark3

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs.

A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).

Language: English
Year: 1999
Pages: 115-123
ISSN: 18732542 and 03781135
Types: Journal article
DOI: 10.1016/S0378-1135(99)00004-8
Keywords

genotyping polymerase chain reaction porcine circovirus wasting syndromes

Other keywords

Animals Circoviridae Infections Circovirus DNA Primers Denmark Genotype Northern Ireland Polymerase Chain Reaction Spain Swine Swine Diseases Wasting Syndrome

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