Journal article
Quantitative Label-Free Cell Proliferation Tracking with a Versatile Electrochemical Impedance Detection Platform
Department of Micro- and Nanotechnology, Technical University of Denmark1
Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark2
Polytechnic University of Milan3
Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark4
Since the use of impedance measurements for label-free monitoring of cells has become widespread but still the choice of sensing configuration is not unique though crucial for a quantitative interpretation of data, we demonstrate the application of a novel custom multipotentiostat platform to study optimal detection strategies.
Electrochemical Impedance Spectroscopy (EIS) has been used to monitor and compare adhesion of different cell lines. HeLa cells and 3T3 fibroblasts have been cultured for 12 hours on interdigitated electrode arrays integrated into a tailor-made cell culture platform. Both vertical and coplanar interdigitated sensing configuration approaches have been used and compared on the same cell populations.
Language: | English |
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Publisher: | IOP Publishing |
Year: | 2012 |
Pages: | 012029 |
ISSN: | 17426596 and 17426588 |
Types: | Journal article |
DOI: | 10.1088/1742-6596/407/1/012029 |
ORCIDs: | Heiskanen, Arto , Andresen, T L and Emnéus, Jenny |