Journal article
Characterization of the Chemoenzymatic Synthesis of N-Acetyl-d-neuraminic Acid (Neu5Ac)
The Advanced Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, U.K.1
Bioprocessing Unit, Glaxo Wellcome Medicines Research Centre, Stevenage, Herts SG1 2NY, U.K.2
The characterization of the chemoenzymatic synthesis of N-acetyl-d-neuraminic acid using a base-catalyzed epimerization and Neu5Ac aldolase-catalyzed condensation is presented. The characterization has been used to exemplify a structured approach to biotransformation process design. In addition, the option of integration of the epimerization with the biotransformation (at alkaline pH) was considered.
Process operating windows have been generated as a result of this approach, defining possible operating regions for subsequent scale-up.
| Language: | English |
|---|---|
| Publisher: | American Chemical Society |
| Year: | 1996 |
| Pages: | 758-763 |
| ISSN: | 87567938 and 15206033 |
| Types: | Journal article |
| DOI: | 10.1021/bp9600647 |
