Journal article
Characterisation of a pestivirus isolated from persistently infected mousedeer (Tragulus javanicus)
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark1
Division of Virology, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Virology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5
Serum samples from the male Mousedeer A and the mother, father and sister of A were tested for bovine virus diarrhoea viruses (BVDV) by isolation, and for BVDV antibodies by blocking ELISA and homologous neutralisation test. Further, RNA was extracted and tested by RT-PCR protocol analysing the 5'-untranslated region and the E2 gene of pestivirus.
The RT-PCR products were subsequently sequenced. Mousedeer A was positive in virus isolation on three occasions (days 1, 19 and 40) and by RT-PCR. The sister and mother of Mousedeer A were also found virus positive by isolation and RT-PCR. Mousedeer A, its sister and its mother, all had an antibody neutralisation titer below 10.
The father of A was virus negative but was positive in the blocking antibody ELISA and had a high neutralisation antibody titer. The repeated detection of BVDV in Mousedeer A, the high amount of virus in serum, the lack of antibodies and the virus positive family members documented that the mousedeer were persistently infected with a pestivirus.
The father of A probably had an acute infection resulting in antibodies to pestivirus and viral clearance. Sequence analysis and phylogenetic analysis revealed that the mousedeer pestivirus was closely related to BVDV Type 1f. The existences of persistently infected animals in non-domestic species have great implications for BVDV eradication campaigns in cattle.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2003 |
Pages: | 1455-1463 |
Journal subtitle: | Official Journal of the Virology Division of the International Union of Microbiological Societies |
ISSN: | 14328798 and 03048608 |
Types: | Journal article |
DOI: | 10.1007/s00705-003-0130-9 |
ORCIDs: | 0000-0002-7040-5586 , Rasmussen, Thomas Bruun and Larsen, Lars Erik |
5' Untranslated Regions Animals Antibodies, Viral Antibody ELISA Bovine Virus Diarrhoea Virus Cattle Chronic Disease Diarrhea Virus 1, Bovine Viral Enzyme-Linked Immunosorbent Assay Female Great Implication Infected Mousedeer LifeSciences Male Molecular Sequence Data Pestivirus Infections Reverse Transcriptase Polymerase Chain Reaction Ruminants Sequence Analysis, DNA Viral Clearance Viral Proteins