Journal article
Genetic diversity of Gallibacterium anatis isolates from different chicken flocks
Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock.
A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%.
One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to have a clonal. population structure within natural populations. There was further evidence of clonal lineages, which may have adapted to different sites within the same animal.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 2003 |
Pages: | 2737-2740 |
ISSN: | 1098660x , 00951137 and 1070633x |
Types: | Journal article |
DOI: | 10.1128/JCM.41.6.2737-2740.2003 |