Book chapter · Journal article
VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants
In
Methods in Molecular Biology
—
2015, Volume 1287, pp. 255-265
Abstract
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
| Language: | English |
|---|---|
| Publisher: | Springer New York |
| Year: | 2015 |
| Pages: | 255-265 |
| Series: | Methods in Molecular Biology |
| Journal subtitle: | Methods and Protocols |
| ISBN: | 1493924524 , 1493924532 , 9781493924523 and 9781493924530 |
| ISSN: | 10643745 and 19406029 |
| Types: | Book chapter and Journal article |
| DOI: | 10.1007/978-1-4939-2453-0_19 |
