Journal article
Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica
An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography.
YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k(cat)/K(m)=19.3s(-1)μM(-1)) and 2,6-dimethoxyphenol (k(cat)/K(m)=13s(-1)μM(-1)) than any other reported laccase.
This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries.
Language: | English |
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Year: | 2012 |
Pages: | 636-45 |
ISSN: | 18732976 and 09608524 |
Types: | Journal article |
DOI: | 10.1016/j.biortech.2012.07.066 |
Amino Acid Sequence Biodegradation, Environmental Carbohydrate Metabolism Cellulase Enzyme Activation Enzyme Inhibitors Evolution, Molecular Hydrolysis Ions Kinetics Laccase Metals Molecular Sequence Data Oryza Peptides Phenols Phylogeny Reproducibility of Results Substrate Specificity Waste Products Yarrowia