Journal article
A multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-γ(+) CMI responses protects against a genital infection in minipigs
Chlamydia is the most widespread sexually transmitted bacterial disease and a prophylactic vaccine is highly needed. Ideally, this vaccine is required to induce a combined response of Th1 cell-mediated immune (CMI) response in concert with neutralizing antibodies. Using a novel Göttingen minipig animal model, we evaluated the immunogenicity and efficacy of a multi-subunit vaccine formulated in the strong Th1-inducing adjuvant CAF01.
We evaluated a mixture of two fusion proteins (Hirep1 and CTH93) designed to promote either neutralizing antibodies or cell-mediated immunity, respectively. Hirep1 is a novel immunogen based on the variant domain (VD) 4 region from major outer membrane protein (MOMP) serovar (Sv) D, SvE and SvF, and CTH93 is a fusion molecule of three antigens (CT043, CT414 and MOMP).
Pigs were immunized twice intramuscularly with either Hirep1+CTH93/CAF01, UV-inactivated Chlamydia trachomatis SvD bacteria (UV-SvD/CAF01) or CAF01. The Hirep1+CTH93/CAF01 vaccine induced a strong CMI response against the vaccine antigens and high titers of antibodies, particularly against the VD4 region of MOMP.
Sera from Hirep1+CTH93/CAF01 immunized pigs neutralized C. trachomatis SvD and SvF infectivity in vitro. Both Hirep1+CTH93/CAF01 and UV-SvD/CAF01 vaccination protected pigs against a vaginal C. trachomatis SvD infection. In conclusion, the Hirep1+CTH93/CAF01 vaccine proved highly immunogenic and equally protective as UV-SvD/CAF01 showing promise for the development of a subunit vaccine against Chlamydia.
Language: | English |
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Publisher: | Nature Publishing Group |
Year: | 2016 |
Pages: | 185-195 |
ISSN: | 14401711 and 08189641 |
Types: | Journal article |
DOI: | 10.1038/icb.2015.79 |
ORCIDs: | 0000-0003-1653-4552 and Jungersen, Gregers |
Animals Antibodies, Neutralizing Bacterial Outer Membrane Proteins Bacterial Vaccines Chlamydia Chlamydia Infections Disease Models, Animal Humans Immunity, Cellular Immunization Interferon-gamma Journal Article Recombinant Fusion Proteins Research Support, Non-U.S. Gov't Swine Swine, Miniature Th1 Cells Vaccines, Inactivated Vaccines, Subunit major outer membrane protein, Chlamydia pneumoniae