Journal article
Response of Substituted Indoleacetic Acids in the Indolo-alpha-pyrone Fluorescence Determination
In
Physiologia Plantarum
—
1978, Volume 43, Issue 1, pp. 62-64
Abstract
The method of indolo-.alpha.-pyrone fluorescence-determination of IAA was investigated to study possible interference from 4-chloro-indoleacetic acid and 5-hydroxyindoleacetic acid, which occur naturally. Both compounds show about 40% of the fluorescence of IAA after conversion into their .alpha.-pyrones.
Other halogenated indoleacetic acids show between zero and 60% of the fluorescence of IAA. Apparently the concentration of IAA cannot be determined in crude extracts in the presence of 4-chloro- or 5-hydroxy-indoleacetic acid, because separate determinations of each of these compounds are not possible by changing the excitation or fluorescence wave-lengths of the testing equipment.
| Language: | English |
|---|---|
| Year: | 1978 |
| Pages: | 62-64 |
| ISSN: | 13993054 and 00319317 |
| Types: | Journal article |
| DOI: | 10.1111/j.1399-3054.1978.tb01567.x |
