Journal article
Recombination among multiple mitochondrial pseudogenes from a passerine genus
PCR products of a fragment of the mitchondrial protein coding subunit 5 of NADH-dehydrogenase (ND5) from eight individuals representing five species of the South American bird genus Conirostrum were cloned. The 130 clones, which were subsequently sequenced, constituted 55 different sequences. Due to the observed differences in substitution patterns 58% of the cloned sequences were identified as pseudogenes.
Recombination could be traced in 19% of the inferred nuclear pseudogenes, but this figure probably represents a Significant underestimation of the factual recombination events. The nonrecombined pseudogenes consisted of multiple haplotypes found to diverge from 1 to 16% from the mitochondrial gene. The number of mitochondrial nuclear copies and their apparent frequent recombination suggest that pseudogenes constitute a serious potential risk in confounding phylogenetic studies and population genetic analysis.
Language: | English |
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Year: | 2001 |
Pages: | 362-369 |
ISSN: | 10959513 and 10557903 |
Types: | Journal article |
DOI: | 10.1006/mpev.2000.0885 |
Amino Acid Sequence Animals Consensus Sequence DNA, Mitochondrial Evolution, Molecular Likelihood Functions Molecular Sequence Data NADH Dehydrogenase Phylogeny Polymerase Chain Reaction Protein Subunits Pseudogenes Recombination, Genetic Sequence Alignment Sequence Homology, Amino Acid Songbirds South America