Journal article
High-throughput screening of tick-borne pathogens in Europe
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail1
National Veterinary Institute2
Central Veterinary Institute3
National Veterinary Institute, Technical University of Denmark4
Section for Bacteriology, Pathology and Parasitology, National Veterinary Institute, Technical University of Denmark5
Section for Epidemiology, National Veterinary Institute, Technical University of Denmark6
Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens, a large scale epidemiological study was conducted on 7050 Ixodes ricinus nymphs collected from France, Denmark, and the Netherlands using a powerful new high-throughput approach.
This advanced methodology permitted the simultaneous detection of 25 bacterial, and 12 parasitic species (including; Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Candidatus Neoehrlichia, Coxiella, Francisella, Babesia, and Theileria genus) across 94 samples. We successfully determined the prevalence of expected (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Rickettsia helvetica, Candidatus Neoehrlichia mikurensis, Babesia divergens, Babesia venatorum), unexpected (Borrelia miyamotoi) and rare (Bartonella henselae) pathogens in the three European countries.
Moreover we detected Borrelia spielmanii, Borrelia miyamotoi, Babesia divergens, and Babesia venatorum for the first time in Danish ticks. This surveillance method represents a major improvement in epidemiological studies, able to facilitate comprehensive testing of tick-borne pathogens, and which can also be customized to monitor emerging diseases.
Language: | English |
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Publisher: | Frontiers Media S.A. |
Year: | 2014 |
Pages: | 103 |
ISSN: | 22352988 |
Types: | Journal article |
DOI: | 10.3389/fcimb.2014.00103 |
Animals Computational Biology Denmark France High-Throughput Nucleotide Sequencing High-Throughput Screening Assays Humans Microbiology Molecular Epidemiology Molecular Sequence Data Netherlands Prevalence Public Health Surveillance QR1-502 Real-Time Polymerase Chain Reaction Reproducibility of Results Tick-Borne Diseases Ticks microfluidic analyses molecular epidemiology surveillance tick borne diseases