Journal article
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control : results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control
Friedrich-Loeffler-Institute1
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark2
Division of Virology, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
National Veterinary Research Institute5
Lombardy and Emilia Romagna Experimental Zootechnic Institute6
Animal Health Research Center7
University of Veterinary Medicine Hannover, Foundation8
Federal Veterinary and Agrochemical Research Centre9
National Veterinary Institute10
Institute of Virology and Immunoprophylaxis11
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail12
Central Veterinary Institute13
...and 3 moreThe current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples.
In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions or suboptimal sensitivity with only a single CSFV genotype.
Follow-up actions involved either improvement of suboptimal assays or replacement of specific laboratory assays with the FLI protocol, with or without modifications. In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics.
Language: | English |
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Publisher: | SAGE Publications |
Year: | 2011 |
Pages: | 999-1004 |
ISSN: | 19434936 and 10406387 |
Types: | Journal article |
DOI: | 10.1177/1040638711416849 |
ORCIDs: | Rasmussen, Thomas Bruun |