Journal article
Pediocin PA-1 and a pediocin producing Lactobacillus plantarum strain do not change the HMA rat microbiota
Section for Aquatic Microbiology and Seafood Hygiene, National Institute of Aquatic Resources, Technical University of Denmark1
National Institute of Aquatic Resources, Technical University of Denmark2
National Food Institute, Technical University of Denmark3
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark4
The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut microbiota is important for assessment of consumer risks associated with the use of biopreservative cultures. Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L. plantarum DDEN 12305, or (iii) supernatants of either of these two strains on the composition of the intestinal microbiota of Human Microbiota Associated (HMA) rats were examined by selective cultivation and molecular methods.
The culturable microbiota was in all treatments dominated by lactic acid bacteria and coliforms and no changes in the rat commensal microbiota were detected after ingestion of either of the two L plantarum strains as determined by both culturable methods and molecular methods (DGGE). Both strains were detected in the faeces after ingestion.
Pediocin PA-1 did not mediate changes of the rat microbiota, and a biological assay indicated that the bacteriocin was degraded or inactivated during passage through the intestine.
| Language: | English |
|---|---|
| Year: | 2009 |
| Pages: | 251-257 |
| ISSN: | 18793460 and 01681605 |
| Types: | Journal article |
| DOI: | 10.1016/j.ijfoodmicro.2009.02.003 |
| ORCIDs: | 0000-0003-2151-7802 and Licht, Tine Rask |
