Journal article
A useful assay for transketolase in asymmetric syntheses
A simple and rapid HPLC assay is reported for the measurement and kinetic study of transketolase. The assay consists of monitoring the rate of conversion of β-hydroxypyruvate and glycolaldehyde to L-erythrulose. The assay is also useful in monitoring transketolase catalysed biotransformations for all reactions which utilise β-hydroxypyruvate as a method of driving the reaction to completion.
Language: | English |
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Publisher: | Kluwer Academic Publishers |
Year: | 1996 |
Pages: | 167-172 |
ISSN: | 15736784 and 0951208x |
Types: | Journal article |
DOI: | 10.1007/BF00158940 |