Journal article
Metabolic fate of 13C-labelled polydextrose and impact on the gut microbiome: A triple-phase study in a colon simulator
The present study introduces a novel triple-phase (liquids, solids and gases) approach, which employed uniformly labelled [U-13C] polydextrose (PDX) for the selective profiling of metabolites generated from dietary fiber fermentation in an in vitro colon simulator using human fecal inocula. Employing 13C NMR spectroscopy, [U-13C] PDX metabolism was observed from colonic digest samples.
The major 13C-labelled metabolites generated were acetate, butyrate, propionate, and valerate. In addition to these short-chain fatty acids (SCFAs), 13C-labelled lactate, formate, succinate, and ethanol were detected in the colon simulator samples. Metabolite formation and PDX substrate degradation were examined comprehensively over time (24 and 48 hours).
Correlation analysis between 13C NMR spectra and gas production confirmed the anaerobic fermentation of PDX to SCFAs. In addition, 16S rRNA gene analysis showed that the level of Erysipelotrichaceae was influenced by PDX supplementation and Erysipelotrichaceae level were statistically correlated with SCFA’s formation.
Overall, our study demonstrates a novel approach to link substrate fermentation and microbial function directly in a simulated colonic environment.
Language: | English |
---|---|
Publisher: | American Chemical Society (ACS) |
Year: | 2018 |
Pages: | 1041-1053 |
ISSN: | 15353907 and 15353893 |
Types: | Journal article |
DOI: | 10.1021/acs.jproteome.7b00683 |
ORCIDs: | 0000-0002-9292-3595 , Meier, Sebastian , 0000-0002-1882-5321 and 0000-0002-8809-9513 |
13C-labeled metabolites Anaerobiosis Bioreactors Biotransformation C NMR C-labelled metabolites Carbon Isotopes Colon Dietary Fiber Erysipelothrix Ethanol Fatty Acids, Volatile Feces Fermentation Formates GUT MICROBIOME Gastrointestinal Microbiome Glucans Humans Lactic Acid Magnetic Resonance Spectroscopy Metabolome Microbial Consortia RNA, Ribosomal, 16S Succinic Acid dietary fiber formic acid gut microbiome polydextrose