Journal article
Application of microbioreactors in fermentation process development: a review
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Department of Chemical and Biochemical Engineering, Technical University of Denmark2
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3
Department of Systems Biology, Technical University of Denmark4
Biotechnology process development involves strain testing and improvement steps aimed at increasing yields and productivity. This necessitates the high-throughput screening of many potential strain candidates, a task currently mainly performed in shake flasks or microtiter plates. However, these methods have some drawbacks, such as the low data density (usually only end-point measurements) and the lack of control over cultivation conditions in standard shake flasks.
Microbioreactors can offer the flexibility and controllability of bench-scale reactors and thus deliver results that are more comparable to large-scale fermentations, but with the additional advantages of small size, availability of online cultivation data and the potential for automation. Current microbioreactor technology is analyzed in this review paper, focusing on its industrial applicability, and directions for future research are presented.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2009 |
Pages: | 679-695 |
ISSN: | 16182650 and 16182642 |
Types: | Journal article |
DOI: | 10.1007/s00216-009-2955-x |
ORCIDs: | Eliasson Lantz, Anna and Gernaey, Krist |
Analytical Chemistry Bacteria Biochemistry, general Bioprocess monitoring Bioreactors Chemistry Environmental Monitoring/Analysis Equipment Design Fermentation Food Science Laboratory Medicine Measurement Science and Instrumentation Microbioreactor Microfluidic Analytical Techniques Microfluidic suspension culture Miniaturization