Journal article
Analysis of the acute phase responses of Serum Amyloid A, Haptoglobin and Type 1 Interferon in cattle experimentally infected with foot-and-mouth disease virus serotype O
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark1
Division of Virology, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Innate Immunology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark5
A series of challenge experiments were performed in order to investigate the acute phase responses to foot-and-mouth disease virus (FMDV) infection in cattle and possible implications for the development of persistently infected "carriers". The host response to infection was investigated through measurements of the concentrations of the acute phase proteins (APPs) serum amyloid A (SAA) and haptoglobin (HP), as well as the bioactivity of type 1 interferon (IFN) in serum of infected animals.
Results were based on measurements from a total of 36 infected animals of which 24 were kept for observational periods exceeding 28 days in order to determine the carrier-status of individual animals. The systemic host response to FMDV in infected animals was evaluated in comparison to similar measurements in sera from 6 mock-inoculated control animals.There was a significant increase in serum concentrations of both APPs and type 1 IFN in infected animals coinciding with the onset of viremia and clinical disease.
The measured parameters declined to baseline levels within 21 days after inoculation, indicating that there was no systemically measurable inflammatory reaction related to the carrier state of FMD. There was a statistically significant difference in the HP response between carriers and non-carriers with a lower response in the animals that subsequently developed into FMDV carriers.
It was concluded that the induction of SAA, HP and type 1 IFN in serum can be used as markers of acute infection by FMDV in cattle.
| Language: | English |
|---|---|
| Publisher: | BioMed Central |
| Year: | 2011 |
| Pages: | 66 |
| ISSN: | 12979716 and 09284249 |
| Types: | Journal article |
| DOI: | 10.1186/1297-9716-42-66 |
| ORCIDs: | Belsham, Graham , Heegaard, Peter M. H. and Stockmarr, Anders |
Acute Phase Protein Acute Phase Response Acute-Phase Proteins Acute-Phase Proteins/genetics Animals Antibodies, Viral Antibodies, Viral/blood Bovine Respiratory Syncytial Virus Carrier State Carrier State/immunology Cattle Contact Animal Enzyme-Linked Immunosorbent Assay Enzyme-Linked Immunosorbent Assay/veterinary Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Virus/immunology Foot-and-Mouth Disease/immunology Haptoglobins Haptoglobins/genetics Immunity, Innate Infected Animal Interferon Type I Interferon Type I/genetics Male Polymerase Chain Reaction Polymerase Chain Reaction/veterinary SF600-1100 Serum Amyloid A Protein Serum Amyloid A Protein/genetics Veterinary medicine Viremia Viremia/immunology
