Journal article · Conference paper
Microchip Flow Cytometer with Integrated Polymer Optical Elements for Measurement of Scattered Light
Flow cytometry is a very powerful method for biophysical measurement of microparticles, such as cells and bacteria. In this paper, we report an innovative microsystem, in which several different optical elements (waveguides, lenses and fiber-to-waveguide couplers) are integrated with microfluidic channels to form a complete microchip flow cytometer.
All the optical elements, the microfluidic system, and the fiber-to-waveguide couplers were defined in one layer of polymer (SU-8, negative photoresist) by standard photolithography. With only one single mask procedure, all the fabrication and packaging processes can be finished in one day. Polystyrene beads were measured in the microchip flow cytometer, and three signals (forward scattering, large angle scattering and extinction) were measured simultaneously for each bead.
The average intensities of the forward Scattered light and the incident light extinction showed excellent linear relationship with the sizes of the beads. To our knowledge this is the first time forward scattered light and incident light extinction were measured in a microsystem using integrated optics.
The microsystem can be applied for analyzing different kinds of particles and cells, and can easily be integrated with other microfluidic components.
| Language: | English |
|---|---|
| Year: | 2004 |
| Pages: | 367-370 |
| Proceedings: | 17th IEEE International Conference on Micro Electro Mechanical Systems. Maastricht MEMS 2004 Technical Digest |
| ISBN: | 078038265X , 078038265x and 9780780382657 |
| ISSN: | 10846999 |
| Types: | Journal article and Conference paper |
| DOI: | 10.1109/MEMS.2004.1290598 |
| ORCIDs: | Wolff, Anders |
Biomedical optical imaging Fluid flow measurement Image motion analysis Integrated optics Light scattering Microfluidics Optical fiber couplers Optical polymers Optical scattering Particle scattering bacteria biophysical measurement cellular biophysics chip scale packaging fiber-waveguide couplers integrated optics integrated polymer optical elements lenses masks micro-optics microchip flow cytometer microfluidic channels microfluidics microorganisms microparticles microsystem optical elements optical fibre couplers optical polymers packaging processes patient diagnosis photolithography photoresist polymer polymer films polystyrene beads scattered light measurement
