Journal article
340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays
We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay.
The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry.
Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.
Language: | English |
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Year: | 2016 |
Pages: | 22135-22143 |
ISSN: | 10944087 |
Types: | Journal article |
DOI: | 10.1364/OE.24.022135 |
ORCIDs: | Rodenko, Olga , Tidemand-Lichtenberg, Peter , Petersen, Paul Michael and Pedersen, Christian |