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Journal article

Optimized localization analysis for single-molecule tracking and super-resolution microscopy

By Mortensen, Kim1,2,3; Churchman, L. S.; Spudich, J. A.; Flyvbjerg, Henrik1,2,3

From

Stochastic Systems and Signals Group, Theory Section, Department of Micro- and Nanotechnology, Technical University of Denmark1

Theory Section, Department of Micro- and Nanotechnology, Technical University of Denmark2

Department of Micro- and Nanotechnology, Technical University of Denmark3

We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula for its precision exaggerates beyond Fisher's information limit, and weighted least-squares may do worse, whereas maximum-likelihood fitting is practically optimal.

Language: English
Publisher: Nature Publishing Group US
Year: 2010
Pages: 377-381
Journal subtitle: Techniques for Life Scientists and Chemists
ISSN: 15487105 and 15487091
Types: Journal article
DOI: 10.1038/NMETH.1447
ORCIDs: Mortensen, Kim and Flyvbjerg, Henrik
Keywords

Image Interpretation, Computer-Assisted Likelihood Functions Microscopy, Fluorescence Nanotechnology Photons

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