Journal article
Synthesis and characterization of an in situ forming hydrogel using tyramine conjugated high methoxyl gum tragacanth
Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.1
Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran evf@modares.ac.ir.2
Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran.3
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Nanotechnology, Stem Cell Technology Research Center, Tehran, Iran.4
In this study, an enzyme catalyzed in situ forming hydrogel based on tyramine conjugated high methoxyl content gum tragacanth (TA-HMGT) was prepared and characterized. TA-HMGT was synthesized via heterogeneous ammonolysis of methyl ester groups of HMGT. Then, the hydrogel was prepared via horseradish peroxidase catalyzed coupling reaction in the presence of hydrogen peroxide.
Hydrogel properties, such as gelation time, swelling/degradation behavior and rheological properties could be adjusted by tuning the gelation parameters and extent of tyramine conjugation. This system was a soft elastic hydrogel with appropriate biocompatibility. The fast gelation of the hydrogel is desirable for clinical applications.
Also, in vitro bovine serum albumin release from the synthesized hydrogel showed good release profile with limited burst release.
| Language: | English |
|---|---|
| Publisher: | SAGE Publications |
| Year: | 2016 |
| Pages: | 1016-1025 |
| ISSN: | 15308022 and 08853282 |
| Types: | Journal article |
| DOI: | 10.1177/0885328215608983 |
Animals Astragalus Plant Biocompatible Materials Caco-2 Cells Cattle Cell Survival Cross-Linking Reagents Drug Delivery Systems Elasticity Esters Horseradish Peroxidase Humans Hydrogels Hydrogen Peroxide In situ forming hydrogel Mesenchymal Stem Cells Rheology Serum Albumin, Bovine Soft Tissues and Materials Tragacanth Tyramine ammonolysis enzymatic gelation high methoxyl tragacanth tyramine
