Journal article
Detection of Avian Influenza Virus by Fluorescent DNA Barcode-based Immunoassay with Sensitivity Comparable to PCR
BioLabChip Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark4
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark5
National Veterinary Institute, Technical University of Denmark6
In this paper, a coupling of fluorophore-DNA barcode and bead-based immunoassay for detecting avian influenza virus (AIV) with PCR-like sensitivity is reported. The assay is based on the use of sandwich immunoassay and fluorophore-tagged oligonucleotides as representative barcodes. The detection involves the sandwiching of the target AIV between magnetic immunoprobes and barcode-carrying immunoprobes.
Because each barcode-carrying immunoprobe is functionalized with a multitude of fluorophore-DNA barcode strands, many DNA barcodes are released for each positive binding event resulting in amplification of the signal. Using an inactivated H16N3 AIV as a model, a linear response over five orders of magnitude was obtained, and the sensitivity of the detection was comparable to conventional RT-PCR.
Moreover, the entire detection required less than 2 hr. The results indicate that the method has great potential as an alternative for surveillance of epidemic outbreaks caused by AIV, other viruses and microorganisms.
| Language: | English |
|---|---|
| Publisher: | The Royal Society of Chemistry |
| Year: | 2010 |
| Pages: | 337-342 |
| ISSN: | 13645528 and 00032654 |
| Types: | Journal article |
| DOI: | 10.1039/b916821b |
| ORCIDs: | Bang, Dang Duong and Wolff, Anders |
