Journal article
Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality
Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr).
Since the mechanisms mediating VK effects over skeletal development are poorly known, we investigated the effects of VK-supplementation on skeletal development in Senegalese sole larvae, aiming to identify molecular pathways involved. Larvae were fed live preys enriched with graded levels of phylloquinone (PK) (0, 50, and 250 mg kg(-1)) and survival rate, growth, VK contents, calcium content and incidence of skeletal deformities were determined, revealing an improvement of larval performance and decreasing the incidence of deformities in VK-supplemented groups.
Comparative proteome analysis revealed a number of differentially expressed proteins between Control and Diet 250 associated with key biological processes including skin, muscle, and bone development. Expression analysis showed that genes encoding proteins related to the VK cycle (ggcx, vkor), VK nuclear receptor (pxr), and VK-dependent proteins (VKDPs; oc1 and grp), were differentially expressed.
This study highlights the potential benefits of increasing dietary VK levels in larval diets, and brings new insights on the mechanisms mediating the positive effects observed on larval performance and skeletal development.
Language: | English |
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Publisher: | Springer US |
Year: | 2014 |
Pages: | 522-537 |
Journal subtitle: | An International Journal Focusing on Marine Genomics, Molecular Biology and Biotechnology |
ISSN: | 14362236 , 14362228 and 14350637 |
Types: | Journal article |
DOI: | 10.1007/s10126-014-9571-2 |
ORCIDs: | Wulff, Tune |
ATLANTIC SALMON BIOTECHNOLOGY Flatfish larvae GLA-RICH PROTEIN Gene expression HASH(0x3cc4408) HEAVY-CHAIN GENES MARINE MOLECULAR CHARACTERIZATION MUMMICHOG FUNDULUS-HETEROCLITUS NICOTINAMIDE BISULFITE VITAMIN-K-3 Proteomics REAL-TIME PCR SALMO-SALAR L. SDG 14 - Life Below Water Skeletal deformities Solea senegalensis VASCULAR CALCIFICATION Vitamin K XENOBIOTIC RECEPTOR SXR