Journal article
Repeated inhalations of diesel exhaust particles and oxidatively damaged DNA in young oxoguanine DNA glycosylase (OGG1) deficient mice
Department of Occupational and Environmental Health, Institute of Public Health, University of Copenhagen, Copenhagen, Denmark. l.risom@pubhealth.ku.dk1
DNA repair may prevent increased levels of oxidatively damaged DNA from prolonged oxidative stress induced by, e.g. exposure to diesel exhaust particles (DEP). We studied oxidative damage to DNA in broncho-alveolar lavage cells, lungs, and liver after 4 x 1.5 h inhalations of DEP (20 mg/m3) in Ogg1-/- and wild type (WT) mice with similar extent of inflammation.
DEP exposure increased lung levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in Ogg1-/- mice, whereas no effect on 8-oxodG or oxidized purines in terms of formamidopyrimidine DNA glycosylase (FPG) sites was observed in WT mice. In both unexposed and exposed Ogg1-/- mice the level of FPG sites in the lungs was 3-fold higher than in WT mice.
The high basal level of FPG sites in Ogg1-/- mice probably saturated the assay and prevented detection of DEP-generated damage. In conclusion, Ogg1-/- mice have elevated pulmonary levels of FPG sites and accumulate genomic 8-oxodG after repeated inhalations of DEP.
| Language: | English |
|---|---|
| Year: | 2007 |
| Pages: | 172-181 |
| ISSN: | 10715762 and 10292470 |
| Types: | Journal article |
| DOI: | 10.1080/10715760601024122 |
| ORCIDs: | Møller, Peter and Loft, Steffen |
8-Hydroxy-2'-Deoxyguanosine Administration, Inhalation Age Factors Animals Body Weight Bronchoalveolar Lavage Fluid DNA Damage DNA Glycosylases DNA Repair DNA-Binding Proteins DNA-Formamidopyrimidine Glycosylase Deoxyguanosine Endonucleases Ercc1 protein, mouse Escherichia coli Proteins Female Heme Oxygenase-1 Hmox1 protein, mouse Interleukin-6 Liver Lung Macrophages, Alveolar Membrane Proteins Mice Mice, Inbred C57BL Mice, Knockout Neutrophils Ogg1 protein, mouse Oxidative Stress Pyrrolidines Quinolizines RNA, Messenger Ro 19-8022 Vehicle Emissions
