Conference paper
Integration of agglutination assay for protein detection in microfluidic disc using Blu-ray optical pickup unit and optical fluid scanning
Department of Micro- and Nanotechnology, Technical University of Denmark1
Nanoprobes, Department of Micro- and Nanotechnology, Technical University of Denmark2
Magnetic Systems, Department of Micro- and Nanotechnology, Technical University of Denmark3
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark4
We present a novel strategy for thrombin detection by combining a magnetic bead based agglutination assay and low-cost microfluidic disc. The detection method is based on an optomagnetic readout system implemented using a Blu-ray optical pickup unit (OPU) as main optoelectronic component. The assay, from sample to answer, is fully integrated on a microfluidic disc which embeds on-disc mixing ensuring full automation of the assay along with less sample-to-answer time compared to similar methods.
Moreover, we compare the optomagnetic readout to the cluster size distribution determined using a commercial optical scanning imaging instrument.
| Language: | English |
|---|---|
| Year: | 2015 |
| Pages: | 1807-1809 |
| Proceedings: | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Types: | Conference paper |
| ORCIDs: | Hansen, Mikkel Fougt and Boisen, Anja |
