Journal article
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria
National Food Institute, Technical University of Denmark1
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark2
Department of Systems Biology, Technical University of Denmark3
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark4
Novo Nordisk Foundation5
Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria ia is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp.
This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 1998 |
Pages: | 2240-2246 |
ISSN: | 10985336 and 00992240 |
Types: | Journal article |
DOI: | 10.1128/AEM.64.6.2240-2246.1998 |
ORCIDs: | Sternberg, Claus and Molin, Søren |