Journal article
Recent Advances in Re-engineering Modular PKS and NRPS Assembly Lines
Polyketides such as the antibiotic erythromycin or the immunosuppressant rapamycin, and non-ribosomal peptides, such as the antibiotics penicillin or vancomycin, are important classes of natural products. The core of these molecules are biosynthesized by large polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS), respectively.
The modular architecture of these enzymatic assembly lines makes them interesting candidates for synthetic biology approaches. The re-engineering efforts aim to understand the molecular structure, produce new compounds, produce analogs of known compounds, tag the products or improve activity and/or yield.
Here, we first consider the definition of PKS and NRPS modules, then give an overview of different strategies for re-engineering and finally review recent examples of PKS and NRPS reengineering.
Language: | English |
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Publisher: | The Korean Society for Biotechnology and Bioengineering |
Year: | 2020 |
Pages: | 886-894 |
ISSN: | 19763816 and 12268372 |
Types: | Journal article |
DOI: | 10.1007/s12257-020-0265-5 |
ORCIDs: | Beck, Charlotte and Weber, Tilmann |