Journal article
Divergent Response Profile in Activated Cord Blood T cells from First-born Child Implies Birth-order-associated in Utero Immune Programming
Background: First-born children are at higher risk for development of a range of immune-mediated diseases. The underlying mechanism of ‘birth-order-effects’ on disease risk is largely unknown, but in utero programming of the child's immune system may play a role. Objective: We studied the association between birth-order and the functional response of stimulated cord blood T cells.
Method: Purified cord blood T cells were polyclonally activated with anti-CD3/CD28-coated beads in a subgroup of 28 children enrolled in the COPSAC2010 birth cohort. Expression levels of seven activation markers on helper and cytotoxic T cells as well as the percentage of CD4+CD25+ T cells were assessed by flow cytometry.
Production of IFN-γ, TNF-α, IL-17, IL-4, IL-5, IL-13 and IL-10 was measured in supernatants. Results: IL-10 secretion (P = 0.007) and CD25 expression on CD4+ helper T cells (P = 0.0003) in activated cord blood T cells were selectively reduced in first-born children, while the percentage of CD4+CD25+ cord blood T cells was independent of birth-order.
Conclusion: First-born infants display a reduced anti-inflammatory profile in T cells at birth. This possible in utero ‘birth-order’ T cell programing may contribute to later development of immune-mediated diseases by increasing overall immune reactivity in first-born children as compared to younger siblings.
Language: | English |
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Year: | 2016 |
Pages: | 323-32 |
ISSN: | 13989995 and 01054538 |
Types: | Journal article |
DOI: | 10.1111/all.12799 |
ORCIDs: | 0000-0003-4131-7592 , Larsen, Jeppe Madura and Pedersen, Susanne Brix |
Antigens, CD Biomarkers Cytokines Female Fetal Blood Humans Immune System Diseases Immunophenotyping Infant Infant, Newborn Journal Article Lymphocyte Activation Male Phenotype Pregnancy Prenatal Exposure Delayed Effects Research Support, Non-U.S. Gov't Risk Factors T-Lymphocyte Subsets immune regulation in utero programming neonates parity number