Journal article
Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology
Department of Micro- and Nanotechnology, Technical University of Denmark1
Nanoprobes, Department of Micro- and Nanotechnology, Technical University of Denmark2
Surface Engineering, Department of Micro- and Nanotechnology, Technical University of Denmark3
Department of Applied Mathematics and Computer Science, Technical University of Denmark4
Cognitive Systems, Department of Applied Mathematics and Computer Science, Technical University of Denmark5
Academia Sinica Taiwan6
Copenhagen University Hospital Herlev and Gentofte7
In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer.
Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody–antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology.
Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.
Language: | English |
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Year: | 2013 |
Pages: | 444011 |
ISSN: | 13616528 and 09574484 |
Types: | Journal article |
DOI: | 10.1088/0957-4484/24/44/444011 |
ORCIDs: | 0000-0002-5971-4978 , Bache, Michael , Alstrøm, Tommy Sonne and Boisen, Anja |
Antibody Specificity Biomarkers Humans Nanotechnology Optical Storage Devices Optics and Photonics Platelet-Derived Growth Factor Prognosis Quartz Crystal Microbalance Techniques Receptors, Urokinase Plasminogen Activator Solubility Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfhydryl Compounds