Conference paper
A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics
Nano-Bio Integrated Systems Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale [1 – 4].
On the other hand, FACSlyse protocol uses only osmotic pressure to lyse erythrocytes allowing further isolation of leukocytes. This motivated us to develop a novel herringbone based lyser which works on the principle of mixing whole blood with pure water in time controlled manner to lyse erythrocytes osmotically on a chip.
Language: | English |
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Publisher: | IEEE |
Year: | 2009 |
Pages: | 417-420 |
Proceedings: | 15th International Solid-State Sensors, Actuators and Microsystems Conference (Transducers 2009) |
ISBN: | 1424441900 , 1424441927 , 1424441935 , 9781424441907 , 9781424441921 and 9781424441938 |
Types: | Conference paper |
DOI: | 10.1109/SENSOR.2009.5285475 |
ORCIDs: | Dimaki, Maria and Svendsen, Winnie Edith |
Blood Processing Cells (biology) Design optimization FACSlyse protocol Fractionation Laser ablation Leukocytes Isolation Microfluidics Mixers Nanoscale devices Nanotechnology Optical device fabrication Prototypes Rapid prototyping White blood cells bioMEMS blood cell sorting cellular biophysics erythrocytes lab-on-a-chip lab-on-chip system leukocyte isolation microfluidics mixing osmosis osmotic pressure passive microfluidic device patient diagnosis point-of-care diagnostics red blood cell lysis whole blood preprocessing whole blood-pure water mixing principle