Journal article
Light-Induced Local Heating for Thermophoretic Manipulation of DNA in Polymer Micro- and Nanochannels
NSE-Optofluidics Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Polymer Microsystems for Cell Processing Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
We present a method for making polymer chips with a narrow-band near-infrared absorber layer that enables light-induced local heating of liquids inside fluidic micro- and nanochannels fabricated by thermal imprint in polymethyl methacrylate. We have characterized the resulting liquid temperature profiles in microchannels using the temperature dependent fluorescence of the complex [Ru(bpy)3]2+.
We demonstrate thermophoretic manipulation of individual YOYO-1 stained T4 DNA molecules inside micro- and nanochannels.
Language: | English |
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Year: | 2010 |
Pages: | 826-832 |
ISSN: | 15306992 and 15306984 |
Types: | Journal article |
DOI: | 10.1021/nl903190q |
ORCIDs: | Thamdrup, Lasse Højlund , Larsen, Niels Bent and Kristensen, Anders |