Journal article
Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing
Mikomed Sp. z o. o.1
SILMEDIC Medical Centre Sp. z o.o.2
Medservice3
University of Medical Sciences Poznan4
Instytut Edukacji i.e. sp.z o.o.5
ClinMed Pharma6
Cell Diversity Lab, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark7
DTU Proteomics Core, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark8
University of Cologne9
Protease Systems Biology, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark10
Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark11
Department of Biotechnology and Biomedicine, Technical University of Denmark12
ERCAN Wound Care Centre13
Professional Treatment and Research Centre14
Medical University of Warsaw15
Pratia Ostrołęka Embedded Hospital16
Przychodnia Wilmed17
...and 7 moreVenous leg ulcers represent a clinical challenge and impair the quality of life of patients. This study examines impaired wound healing in venous leg ulcers at the molecular level. Protein expression patterns for biomarkers were analysed in venous leg ulcer wound fluids from 57 patients treated with a protease-modulating polyacrylate wound dressing for 12 weeks, and compared with exudates from 10 acute split-thickness wounds.
Wound healing improved in the venous leg ulcer wounds: 61.4% of the patients with venous leg ulcer achieved a relative wound area reduction of ≥ 40%, and 50.9% achieved a relative wound area reduction of ≥ 60%. Within the first 14 days, abundances of S100A8, S100A9, neutrophil elastase, matrix metalloproteinase-2, and fibronectin in venous leg ulcer exudates decreased significantly and remained stable, yet higher than in acute wounds.
Interleukin-1β, tumour necrosis factor alpha, and matrix metalloproteinase-9 abundance ranges were similar in venous leg ulcers and acute wound fluids. Collagen (I) α1 abundance was higher in venous leg ulcer wound fluids and was not significantly regulated. Overall, significant biomarker changes occurred in the first 14 days before a clinically robust healing response in the venous leg ulcer cohort.
Language: | English |
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Publisher: | Medical Journals Sweden |
Year: | 2022 |
Pages: | adv00834 |
ISSN: | 16512057 and 00015555 |
Types: | Journal article |
DOI: | 10.2340/actadv.v102.325 |
ORCIDs: | Kalogeropoulos, Konstantinos , Bundgaard, Louise , Larsen, Cathrine Agnete , Moldt Haack, Aleksander , Schoof, Erwin M. and Auf dem Keller, Ulrich |