Journal article
Gastrointestinal toxicity during induction treatment for childhood acute lymphoblastic leukemia: The impact of the gut microbiota
Copenhagen University Hospital Herlev and Gentofte1
Technical University of Denmark2
Odense University Hospital3
University of Copenhagen4
Aarhus University Hospital5
National Food Institute, Technical University of Denmark6
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark7
Intestinal mucositis is a common side effect of chemotherapy leading to diarrhea, abdominal pain and increased risk of infections. The intestinal microbiota has been recognized as a key regulator of mucosal immune responses. Therefore, we hypothesized that intestinal microbial changes would be associated with enterocyte loss and systemic inflammation during induction treatment for childhood acute lymphoblastic leukemia (ALL).
We prospectively included 51 children newly‐diagnosed with ALL treated in Denmark in 2015–2018. Plasma C‐reactive protein (CRP), plasma citrulline (marker of functional enterocytes mass) measurements and fecal samplings were performed on treatment Days 1, 8, 15, 22 and 29. Moreover, intestinal mucositis was scored by a trained nurse/physician.
Fecal samples in patients and 19 healthy siblings were analyzed by 16S rRNA gene sequencing (V3–V4 region). Bacterial alpha diversity was lower in patients compared to siblings. It decreased from Day 1 to Days 8–22 and increased on Day 29. Shannon alpha diversity index was correlated with CRP on Days 15–29 (rho = −0.33−0.49; p
| Language: | English |
|---|---|
| Publisher: | John Wiley & Sons, Inc. |
| Year: | 2020 |
| Pages: | 1953-1962 |
| ISSN: | 10970215 , 08986924 and 00207136 |
| Types: | Journal article |
| DOI: | 10.1002/ijc.32942 |
| ORCIDs: | 0000-0003-3224-1346 , 0000-0002-1416-8205 , 0000-0001-8121-1114 , 0000-0003-3750-8556 and Pamp, Sünje Johanna |
Adolescent Bacteria C-reactive protein Case-Control Studies Child Child, Preschool C‐reactive protein DNA, Bacterial DNA, Ribosomal Feces Female Gastrointestinal Microbiome Humans Induction Chemotherapy Infant Male Mucositis Phylogeny Precursor Cell Lymphoblastic Leukemia-Lymphoma Prospective Studies RNA, Ribosomal, 16S Sequence Analysis, DNA Siblings acute lymphoblastic leukemia citrulline gastrointestinal toxicity microbiota mucositis
