Journal article
PCR specific for Actinobacillus pleuropneumoniae serotype 3
Imperial College London1
National Veterinary Institute, Technical University of Denmark2
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark3
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
National Research Council of Canada5
University of Bern6
Huazhong Agricultural University7
Royal Veterinary College8
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polisaccharides, but the method has liminations, for example, cross-reactions between serotypes 3, 6, and 8.
This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organism's specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs.
The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6, and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.
Language: | English |
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Year: | 2008 |
Pages: | 648-652 |
ISSN: | 20427670 , 00424900 and 02143909 |
Types: | Journal article |
DOI: | 10.1136/vr.162.20.648 |