Journal article
Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors
The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells.
We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.
Language: | English |
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Year: | 2010 |
Pages: | 7421-7426 |
ISSN: | 19327455 and 19327447 |
Types: | Journal article |
DOI: | 10.1021/jp910034z |