Journal article
Enzymatic polymerization of polythiophene by immobilized glucose oxidase
Department of Physical Chemistry, Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius 03225, Lithuania1
NanoTechnas – Center of Nanotechnology and Materials Science, Faculty of Chemistry, Vilnius University, Naugarduko 24, Vilnius 03225, Lithuania2
Department of Chemistry, Faculty of Science, Selcuk University, Konya 42075, Turkey3
Laboratory of BioNanoTechnology, Department of Material Science and Electrical Engineering, State Research Institute Center for Physical Sciences and Technology, A. Gostauto 11, Vilnius 01108, Lithuania4
In this study ‘green’, environmentally friendly enzymatic reaction-based synthesis of conducting polymer polythiophene (PTP) is proposed. Glucose oxidase (GOx) was shown as an effective catalyst, which, in the presence of glucose, produces hydrogen peroxide suitable for the oxidative polymerization of PTP under ambient conditions at neutral pH.
Enzymatically induced formation of the PTP layer over GOx-modified graphite rod electrode (GRE) was demonstrated and evaluated amperometrically and by attenuated total reflectance – Fourier transform infrared (ATR-FTIR) spectroscopy. Surface morphology of GOx- and PTP-modified GR electrodes was characterized by atomic force microscopy.
It was clearly shown that the apparent kinetic Michaelis constant (KM(app.)) of GOx/PTP-modified GRE increased by increasing the duration of polymerization reaction. Therefore, enzymatic polymerization could be applied in adjustment and/or tuning of KM(app.) and other kinetic parameters of GOx-based electrodes used in biosensor design.
| Language: | English |
|---|---|
| Year: | 2014 |
| Pages: | 1613-1620 |
| ISSN: | 18732291 and 00323861 |
| Types: | Journal article |
| DOI: | 10.1016/j.polymer.2014.02.003 |
