Journal article
Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy
A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane.
This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation.
The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.
Language: | English |
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Year: | 2002 |
Pages: | 29-34 |
ISSN: | 18733468 and 00145793 |
Types: | Journal article |
DOI: | 10.1016/s0014-5793(02)02391-8 |
ORCIDs: | 0000-0002-4258-8960 |
AFM, atomic force microscopy Atomic force microscopy Avidin Biotin DCnPC, saturated di-acyl phosphatidylcholine with n carbon atoms in each acyl chain Langmuir-Blodgett film Langmuir–Blodgett film Ligands Lipid Bilayers Lipid bilayer membrane Lipid domain Lipid membrane heterogeneity Lipid mixture Lipids Macromolecular Substances Membrane Microdomains Membranes, Artificial Microscopy, Atomic Force Phosphatidylcholine Phosphorylcholine