Journal article
Screening the Biosphere: The Fungicolous Fungus Trichoderma phellinicola, a Prolific Source of Hypophellins, New 17-, 18-, 19-, and 20-Residue Peptaibiotics
To investigate the significance of antibiotics for the producing organism(s) in the natural habitat, we screened a specimen of the fungicolous fungus Trichoderma phellinicola (syn. Hypocrea phellinicola) growing on its natural host Phellinus ferruginosus. Results revealed that a particular group of nonribosomal antibiotic polypeptides, peptaibiotics, which contain the non-proteinogenic marker amino acid, a-aminoisobutyric acid, was biosynthesized in the natural habitat by the fungicolous producer and, consequently, released into the host.
By means of liquid chromatography coupled to electrospray highresolution time-of-flight mass spectrometry, we detected ten 20-residue peptaibols in the specimen. Sequences of peptaibiotics found in vivo were independently confirmed by analyzing the peptaibiome of an agar plate culture of T. phellinicola CBS 119283 (ex-type) grown under laboratory conditions.
Notably, this strain could be identified as a potent producer of 39 new 17-, 18-, and 19-residue peptaibiotics, which
Language: | English |
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Publisher: | WILEY-VCH Verlag |
Year: | 2013 |
Pages: | 787-812 |
ISSN: | 16121880 and 16121872 |
Types: | Journal article |
DOI: | 10.1002/cbdv.201200339 |
ORCIDs: | Nielsen, Kristian Fog and Thrane, Ulf |