Journal article
Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production
University of Copenhagen1
Department of Chemical and Biochemical Engineering, Technical University of Denmark2
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark3
Protein Biochemistry Bioscience Center4
University of California at Riverside5
We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH).
All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R-2 = 0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R-2 = 0.4269).
All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform. (C) 2014 Elsevier Ltd.
All rights reserved.
| Language: | English |
|---|---|
| Year: | 2014 |
| Pages: | 261-267 |
| ISSN: | 18732909 and 09619534 |
| Types: | Journal article |
| DOI: | 10.1016/j.biombioe.2014.03.006 |
| ORCIDs: | Jørgensen, Henning , 0000-0002-2233-5122 and 0000-0002-6537-0155 |
00512, General biology - Conservation and resource management 04500, Mathematical biology and statistical methods 10060, Biochemistry studies - General 10068, Biochemistry studies - Carbohydrates 39008, Food microbiology - General and miscellaneous 51522, Plant physiology - Chemical constituents AGRICULTURAL BIOTECHNOLOGY Biochemistry and Molecular Biophysics Bioprocess Engineering CELLULOSIC ETHANOL CORN STOVER Cellulosic ethanol Computational Biology Conservation Denmark Europe Palearctic region ENERGY ENZYMATIC-HYDROLYSIS Enzymatic hydrolysis Genetic selection High throughput screening IMPACT Lignocellulosic ethanol Lignocellulosic ethanols Mathematical Biology Microscale pretreatment Monocotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Monocots, Plants, Spermatophytes, Vascular Plants) - Gramineae [25305] winter wheat common cultivar-Flair, cultivar-Penso, cultivar-Abika, cultivar-Oakley, cultivar-Inspiration, cultivar-Audi, cultivar-Skalmeje, cultivar-Jenga, cultivar-Potenzial, cultivar-Glasgow, cultivar-Dinosor, cultivar-Smuggler, cultivar-Ambition, cultivar-Hattrick, cultivar-Florett, cultivar-Tommi, cultivar-Samyl, cultivar-Tuscan, cultivar-Robigus, cultivar-Opus PRETREATMENT TECHNOLOGIES Pre-Treatment SDG 7 - Affordable and Clean Energy WHEAT-STRAW Wheat straw Wheat straws advanced high throughput pretreatment system laboratory techniques cellulose 9004-34-6 enzymatic hydrolysis system laboratory techniques enzymatic saccharification ethanol 64-17-5 fermentable sugars glucose 58367-01-4 high throughput screening method laboratory techniques lignocellulosic biomass recalcitrant feedstock statistical analysis mathematical and computer techniques xylose 25990-60-7
