Journal article
Continuous Cultivation of Photosynthetic Bacteria for Fatty Acids Production
In the present work, we introduced a novel approach for microbial fatty acids (FA) production. Photosynthetic bacteria, Rhodobacter sphaeroides KD131, were cultivated in a continuous-flow, stirred-tank reactor (CFSTR) at various substrate (lactate) concentrations.At hydraulic retention time (HRT) 4 d, cell concentration continuously increased from 0.97 g dcw/L to 2.05 g dcw/L as lactate concentration increased from 30 mM to 60 mM.
At 70 mM, however, cell concentration fluctuated with incomplete substrate degradation. By installing a membrane unit to CFSTR, a stable performance was observed under much higher substrate loading (lactate 100 mM and HRT 1.5 d). A maximum cell concentration of 16.2 g dcw/L, cell productivity of 1.9 g dcw/L/d, and FA productivity of 665 mg FA/L/d were attained, and these values were comparable with those achieved using microalgae.
The FA content of R. sphaeroides was around 35% of dry cell weight, mainly composed of vaccenic acid (C18:1, omega-7).
Language: | English |
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Year: | 2013 |
Pages: | 277-282 |
ISSN: | 18732976 and 09608524 |
Types: | Journal article |
DOI: | 10.1016/j.biortech.2013.08.078 |