Journal article
Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination
Section of Fish Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark1
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Rainbow trout fingerlings were immunized by intramuscular injection of a plasmid DNA vector encoding the viral haemorrhagic septicaemia virus (VHSV) glycoprotein (G) or nucleocapsid protein (N) genes under the control of a cytomegalovirus promoter. Challenge with VHSV 52 days later demonstrated that both viral genes, and the G gene in particular, were able to induce protective immunity against VHS.
In contrast to sera taken from fish injected with the N gene, neutralizing antibody activity could be detected both before and after challenge in the sera of a major proportion of the fish injected with the G gene.
Language: | English |
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Year: | 1998 |
Pages: | 261-270 |
ISSN: | 10959947 and 10504648 |
Types: | Journal article |
DOI: | 10.1006/fsim.1997.0134 |
ORCIDs: | Lorenzen, Niels |