Journal article
Transfer and effects of 1,2,3,5,7-pentachloronaphthalene in an experimental food chain
ECT Oekotoxikologie GmbH1
University of Bern2
Department of Environmental Engineering, Technical University of Denmark3
Environmental Chemistry, Department of Environmental Engineering, Technical University of Denmark4
Aarhus University5
St. Petersburg State University6
Slovak Medical University7
Goethe University Frankfurt8
Polychlorinated naphthalenes are environmentally relevant compounds that are measured in biota at concentrations in the μg/kg lipid range. Despite their widespread occurrence, literature data on the accumulation and effects of these compounds in aquatic ecosystems are sparsely available. The goal of this study was to gain insights into the biomagnification and effects of 1,2,3,5,7-pentachloronaphthalene (PeCN52) in an experimental food chain consisting of benthic worms and juvenile rainbow trout.
Worms were contaminated with PeCN52 by passive dosing from polydimethylsiloxane silicone. The contaminated worms were then used to feed the juvenile rainbow trout at 0.12, 0.25 or 0.50μg/gfishwet weight/day, and the resulting internal whole-body concentrations of the individual fish were linked to biological responses.
A possible involvement of the cellular detoxification system was explored by measuring PeCN52-induced expression of the phase I biotransformation enzyme gene cyp1a1 and the ABC transporter gene abcb1a. At the end of the 28-day study, biomagnification factors were similar for all dietary intake levels with values between 0.5 and 0.7kglipidfish/kg lipidworm.
The average uptake efficiency of 60% indicated that a high amount of PeCN52 was transferred from the worms to the fish. Internal concentrations of up to 175mg/kg fish lipid in the highest treatment level did not result in effects on survival, behavior, or growth of the juvenile trout, but were associated with the induction of phase I metabolism which was evident from the significant up-regulation of cyp1a1 expression in the liver.
In contrast, no changes were seen in abcb1a transcript levels.
Language: | English |
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Year: | 2015 |
Pages: | 46-54 |
ISSN: | 18781659 and 15320456 |
Types: | Journal article |
DOI: | 10.1016/j.cbpc.2015.01.001 |
ORCIDs: | Mayer, Philipp |
ABCB1 Animals CYP1A1 Cytochrome P-450 CYP1A1 Dietary exposure Dose-Response Relationship, Drug Food Chain Gene Expression Regulation, Enzymologic Juvenile growth test Naphthalenes Oligochaeta Oncorhynchus mykiss Passive dosing Polychlorinated naphthalenes Rainbow trout Water Pollutants, Chemical