Conference paper
Inactivation of vhsv by infiltration and salt under experimental conditions
At the moment the only legal method in Denmark to sanitize wastewater from fish cutting plants is by infiltration. To evaluate the inactivation effect of infiltration on VHSV an experimental examination was initiated. A column packed with gravel as top- and bottom layer (total of 22 cm) and a mid layer consisting of dug sand (76 cm) was used for the trial.
Over a period of 18 h 3.9 x 1010 TCID50 VHSV was supplied to the column, where after normal tap water was supplied for the rest of the trial period, in total 7 days. During the 7 days samples for virological examination were taken. The sampling was most intensive in the period where the risk of VHSV breaking through the column was highest.
The sensitivity of the virological examination was 13.9 TCID50/ml and no virus was isolated. A reduction of VHSV > 4 log in the outlet water was seen. This experiment suggests that infiltration can be a valuable method to sanitize VHSV infected water. Changes in temperature, pH, earth types in the area used for infiltration etc. may change the virus reduction, though.
As some of the fish cutting plants are also smoking rainbow trout fillets, the question arose whether a brine solution will inactivate VHSV. In order to answer this question a small trial was set up. VHSV and NaCl was added to cell culture medium with 10% foetal bovine serum, in order to mimic a “dirty” environment, to obtain from 1.9% to 20.9% NaCl and kept in the dark at 4°C.
Samples were titrated after 5 min, 1 h and 20 h. No reduction in titre was observed in any of the samples.
Language: | English |
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Year: | 2015 |
Pages: | 149-149 |
Proceedings: | 17th International Conference on Diseases of Fish and Shellfish |
Types: | Conference paper |
ORCIDs: | 0000-0002-9489-1419 and Olesen, Niels Jørgen |