Journal article · Book chapter
CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells
CHO Cell Line Engineering and Design, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Ajou University4
The emergence of CRISPR/Cas9 system as a precise and affordable method for genome editing has prompted its rapid adoption for the targeted integration of transgenes in Chinese hamster ovary (CHO) cells. Targeted gene integration allows the generation of stable cell lines with a controlled and predictable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins.
Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.
| Language: | English |
|---|---|
| Year: | 2019 |
| Pages: | 213-232 |
| Series: | Methods in Molecular Biology |
| ISSN: | 19406029 and 10643745 |
| Types: | Journal article and Book chapter |
| DOI: | 10.1007/978-1-4939-9170-9_13 |
| ORCIDs: | Sergeeva, Daria and Kildegaard, Helene Faustrup |
Animals CHO Cells CRISPR-Cas Systems Cricetinae Cricetulus Gene Editing Genome
