Journal article
The Design-Build-Test-Learn cycle for metabolic engineering of Streptomycetes
Natural Products Genome Mining, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
University of California at Berkeley3
Yeast Natural Products, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Synthetic Biology Tools for Yeast, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Center for Microbial Secondary Metabolites, Centers, Technical University of Denmark6
Streptomycetes are producers of a wide range of specialized metabolites of great medicinal and industrial importance, such as antibiotics, antifungals, or pesticides. Having been the drivers of the golden age of antibiotics in the 1950s and 1960s, technological advancements over the last two decades have revealed that very little of their biosynthetic potential has been exploited so far.
Given the great need for new antibiotics due to the emerging antimicrobial resistance crisis, as well as the urgent need for sustainable biobased production of complex molecules, there is a great renewed interest in exploring and engineering the biosynthetic potential of streptomycetes. Here, we describe the Design-Build-Test-Learn (DBTL) cycle for metabolic engineering experiments in streptomycetes and how it can be used for the discovery and production of novel specialized metabolites.
Language: | English |
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Year: | 2021 |
Pages: | 261-275 |
ISSN: | 17441358 and 00711365 |
Types: | Journal article |
DOI: | 10.1042/EBC20200132 |
ORCIDs: | Weber, Tilmann and Whitford, Christopher M |