Journal article
Generation of mutation hotspots in ageing bacterial colonies
CHU, Paris1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Microbial Evolution and Synthetic Biology, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand.
This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2016 |
| Pages: | 2 |
| ISSN: | 20452322 |
| Types: | Journal article |
| DOI: | 10.1038/s41598-016-0005-4 |
| ORCIDs: | 0000-0002-6350-5001 and Nørholm, Morten H. H. |
