Conference paper
Real-Time PCR using a PCR Microchip with Integrated Thermal System and Polymer Waveguides for the Detection of Campylobacter jejuni
In
Proceedings of Ieee Mems 2006
—
2006, pp. 542-545
Abstract
A novel real-time PCR microchip platform with integrated thermal system and polymer waveguides has been developed. By using the integrated optical system of the real-time PCR chip, cadF – a virulence gene of Campylobacter jejuni, could specifically be detected. Two different DNA binding dyes, SYTOX Orange and TO-PRO-3, were added to the PCR mixture to realize the real-time PCR.
The presented approach shows reliable real-time quantitative information of the PCR amplification of the targeted gene.
| Language: | English |
|---|---|
| Publisher: | IEEE |
| Year: | 2006 |
| Pages: | 542-545 |
| Proceedings: | 19th IEEE International Conference on Micro Electro Mechanical Systems |
| ISBN: | 0780394755 and 9780780394759 |
| ISSN: | 10846999 |
| Types: | Conference paper |
| DOI: | 10.1109/MEMSYS.2006.1627856 |
| ORCIDs: | Wolff, Anders |
