Journal article
A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes
East China University of Science and Technology1
Chinese Academy of Agricultural Sciences2
Chinese Academy of Sciences3
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
New Bioactive Compounds, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR–Cas12a.
We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches.
Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.
| Language: | English |
|---|---|
| Year: | 2021 |
| Pages: | 69-77 |
| ISSN: | 20959281 and 20959273 |
| Types: | Journal article |
| DOI: | 10.1016/j.scib.2020.09.004 |
| ORCIDs: | Tong, Yaojun |
Alpha fetoprotein detection Aptamer Biosensing platform CRISPR-Cas12a Cocaine detection Diverse analyte
