Journal article
NanoTIO2 (UV-Titan) does not induce ESTR mutations in the germline of prenatally exposed female mice
National Food Institute, Technical University of Denmark1
Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark2
Environmental Health Science and Research Bureau3
National Research Centre for the Working Environment4
Department of Micro- and Nanotechnology, Technical University of Denmark5
Particulate air pollution has been linked to an increased risk of cardiovascular disease and cancer. Animal studies have shown that inhalation of air particulates induces mutations in the male germline. Expanded simple tandem repeat (ESTR) loci in mice are sensitive markers of mutagenic effects on male germ cells resulting from environmental exposures; however, female germ cells have received little attention.
Oocytes may be vulnerable during stages of active cell division (e.g., during fetal development). Accordingly, an increase in germline ESTR mutations in female mice prenatally exposed to radiation has previously been reported. Here we investigate the effects of nanoparticles on the female germline. Since pulmonary exposure to nanosized titanium dioxide (nanoTiO2) produces a long-lasting inflammatory response in mice, it was chosen for the present study.
Findings Pregnant C57BL/6 mice were exposed by whole-body inhalation to the nanoTiO2 UV-Titan L181 (~42.4 mg UV-Titan/m3) or filtered clean air on gestation days (GD) 8–18. Female C57BL/6 F1 offspring were raised to maturity and mated with unexposed CBA males. The F2 descendents were collected and ESTR germline mutation rates in this generation were estimated from full pedigrees (mother, father, offspring) of F1 female mice (192 UV-Titanexposed F2 offspring and 164 F2 controls).
ESTR mutation rates of 0.029 (maternal allele) and 0.047 (paternal allele) in UV-Titan-exposed F2 offspring were not statistically different from those of F2 controls: 0.037 (maternal allele) and 0.061 (paternal allele). Conclusions We found no evidence for increased ESTR mutation rates in F1 females exposed in utero to UV-Titan nanoparticles from GD8-18 relative to control females.
Language: | English |
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Publisher: | BioMed Central |
Year: | 2012 |
Pages: | 19 |
ISSN: | 17438977 |
Types: | Journal article |
DOI: | 10.1186/1743-8977-9-19 |
<it>In utero</it> Animals DNA Repeat Expansion ESTR Female Germ-Line Mutation HD7260-7780.8 In utero Industrial hygiene. Industrial welfare Inhalation Exposure Male Maternal Exposure Metal Nanoparticles Mice Mice, Inbred C57BL Mice, Inbred CBA Mutagens Nanoparticles Oogenesis Pregnancy RA1190-1270 Repetitive Sequences, Nucleic Acid Titanium Toxicology. Poisons titanium dioxide